6TZT
Crystal structure of human alpha/epsilon-COP of the COPI vesicular coat bound to alpha-COP STM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-29 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9201 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 138.470, 138.470, 192.868 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.624 - 3.065 |
R-factor | 0.18 |
Rwork | 0.178 |
R-free | 0.22250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mkr |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.13-2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.624 | 29.620 | 3.140 |
High resolution limit [Å] | 3.065 | 13.710 | 3.065 |
Rmerge | 0.090 | 0.035 | 0.795 |
Rmeas | 0.094 | 0.037 | 0.839 |
Rpim | 0.029 | 0.012 | 0.265 |
Total number of observations | 4387 | 28290 | |
Number of reflections | 40664 | 471 | 2924 |
<I/σ(I)> | 20.6 | 57.6 | 2.8 |
Completeness [%] | 99.7 | 90.5 | 97.9 |
Redundancy | 10.2 | 9.3 | 9.7 |
CC(1/2) | 0.999 | 0.999 | 0.819 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 300 | 0.1 M sodium citrate, pH 6.0, 1% (v/v) Tacsimate, pH 5.0, 9% (w/v) PEG 3350, 5 mM dithiothreitol Cryoprotected with 30% (v/v) ethylene glycol. |