6TYX
Structure of Ku80 von Willebrand domain S229A mutant complexed with XLF Ku Binding Motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-05-20 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.798, 71.605, 74.600 |
| Unit cell angles | 90.00, 98.31, 90.00 |
Refinement procedure
| Resolution | 40.686 - 1.899 |
| R-factor | 0.186661278414 |
| Rwork | 0.185 |
| R-free | 0.22501 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6tyz |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.647 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Rmeas | 0.050 | 0.335 |
| Number of reflections | 36348 | 1765 |
| <I/σ(I)> | 39.7 | |
| Completeness [%] | 98.6 | |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M HEPES NaOH pH 7.5, 25 % (w/v) PEG3350 |
