6TYE
Crystal structure of MTB sigma L transcription initiation complex with 5 nt long RNA primer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-10 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 129.527, 158.522, 214.656 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.016 - 3.790 |
| R-factor | 0.2367 |
| Rwork | 0.235 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6dvc |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.020 | 3.940 |
| High resolution limit [Å] | 3.790 | 3.830 |
| Number of reflections | 42832 | 3288 |
| <I/σ(I)> | 9.1 | |
| Completeness [%] | 99.0 | |
| Redundancy | 9 | |
| CC(1/2) | 0.879 | 0.879 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 100 mM sodium citrate tribasic dihydrate, pH 5.6, 200 mM sodium acetate, and 10% PEG4000 |
