6TXQ

The high resolution structure of the FERM domain and helical linker of human moesin

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	112.419, 112.419, 61.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	79.492 - 1.730
Rwork	0.200
R-free	0.23300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1e5w
RMSD bond length	0.010
RMSD bond angle	1.560
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.420	1.760
High resolution limit [Å]	1.730	1.730
Rmerge	0.260	16.039
Rmeas	0.262	16.345
Rpim	0.038	3.121
Number of reflections	41463	2210
<I/σ(I)>	15.6	0.7
Completeness [%]	100.0	99.8
Redundancy	88.2	51.5
CC(1/2)	0.999	0.377

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.1M ammonium acetate, 0.1M tris pH 8.5, 32% propan-2-ol