6TXO
Crystal structure of the haemagglutinin mutant (Gln226Leu, Del228) from an H10N7 seal influenza virus isolated in Germany in complex with avian receptor analogue 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.872, 211.996, 82.479 |
| Unit cell angles | 90.00, 105.03, 90.00 |
Refinement procedure
| Resolution | 64.300 - 2.400 |
| R-factor | 0.2503 |
| Rwork | 0.249 |
| R-free | 0.27120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.990 |
| Data reduction software | Aimless |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.480 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 88879 | 4559 |
| <I/σ(I)> | 8.1 | 1 |
| Completeness [%] | 98.6 | |
| Redundancy | 3 | |
| CC(1/2) | 0.996 | 0.454 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 10% PEG6000, 0.1M bicine pH9.0 |
