6TX2
Human HPF1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-18 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 65 |
| Unit cell lengths | 115.099, 115.099, 90.388 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.550 - 2.090 |
| R-factor | 0.1894 |
| Rwork | 0.189 |
| R-free | 0.20730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HPF1 from Nematostella vectensis |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3126) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.680 | 2.170 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Rmerge | 0.170 | 2.670 |
| Number of reflections | 80356 | 7931 |
| <I/σ(I)> | 11.5 | 1.2 |
| Completeness [%] | 99.7 | 98.94 |
| Redundancy | 19.66 | 18.68 |
| CC(1/2) | 1.000 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 33% glycerol ethoxylate, 0.23 M lithium citrate tribasic tetrahydrate |
