6TVV
Crystal structure of 3'-5' RecJ exonuclease from M. Jannaschii
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X13 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X13 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.89113 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 92.070, 92.070, 275.760 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 87.330 - 2.800 |
R-factor | 0.21683 |
Rwork | 0.214 |
R-free | 0.26389 |
Structure solution method | SIRAS |
RMSD bond length | 0.008 |
RMSD bond angle | 1.601 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.900 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.082 | |
Number of reflections | 30267 | 4308 |
<I/σ(I)> | 13.7 | 4.4 |
Completeness [%] | 99.2 | |
Redundancy | 6.9 | |
CC(1/2) | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M Sodium citrate, 0.1 M Bis-Tris-Propane pH 8.5, 14-24 % w/v PEG 3350, 10 mM MnCl2 |