6TVF
Crystal structure of the haemagglutinin from a H10N7 seal influenza virus isolated in Germany in complex with human receptor analogue, 6'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-27 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.815, 214.138, 158.394 |
| Unit cell angles | 90.00, 101.07, 90.00 |
Refinement procedure
| Resolution | 68.520 - 2.600 |
| R-factor | 0.2412 |
| Rwork | 0.239 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.814 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.520 | 2.640 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Number of reflections | 138258 | 6846 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.0 | |
| Redundancy | 3.2 | |
| CC(1/2) | 0.998 | 0.576 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 2%-4% PEG6000, 0.1M HEPES pH7.0 |
