6TVB

Crystal structure of the haemagglutinin from a transmissible H10N7 seal influenza virus isolated in Netherland in complex with human receptor analogue 6'-SLN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-14
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	64.460, 213.110, 76.580
Unit cell angles	90.00, 98.28, 90.00

Refinement procedure

Resolution	54.730 - 1.650
R-factor	0.2014
Rwork	0.201
R-free	0.21500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4d00
RMSD bond length	0.006
RMSD bond angle	1.054
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.730	1.680
High resolution limit [Å]	1.650	1.650
Number of reflections	243953	12134
<I/σ(I)>	8.8	1.1
Completeness [%]	100.0	100
Redundancy	6.7	6.5
CC(1/2)	1.000	0.500

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	26%-31% PEG600, 0.1M HEPES pH 7.5