6TVA
Crystal structure of the haemagglutinin from a transmissible H10N7 seal influenza virus isolated in Netherland in complex with avian receptor analogue, 3'-SLN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.580, 214.960, 77.310 |
| Unit cell angles | 90.00, 98.94, 90.00 |
Refinement procedure
| Resolution | 53.740 - 1.740 |
| R-factor | 0.2016 |
| Rwork | 0.201 |
| R-free | 0.21530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d00 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.051 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.740 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmeas | 0.117 | |
| Number of reflections | 211775 | 10548 |
| <I/σ(I)> | 9.6 | 1.4 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 6.7 | |
| CC(1/2) | 1.000 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 26%-31% PEG600,0.1M HEPES pH 7.5 or 0.1 M tris pH 8.0 |
