6TV4
CFF-Notum complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.96859 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 59.987, 71.574, 78.313 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.830 - 1.530 |
R-factor | 0.1725 |
Rwork | 0.170 |
R-free | 0.21420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6r8p |
RMSD bond length | 0.004 |
RMSD bond angle | 1.233 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.830 | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Number of reflections | 51294 | 2518 |
<I/σ(I)> | 14.3 | |
Completeness [%] | 99.4 | |
Redundancy | 12.7 | |
CC(1/2) | 0.990 | 0.480 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 300 | 1.5 M Ammonium Sulphate 0.1 M Sodium citrate pH 4.2 |