6TV4
CFF-Notum complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.96859 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.987, 71.574, 78.313 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.830 - 1.530 |
| R-factor | 0.1725 |
| Rwork | 0.170 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6r8p |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.233 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.830 | 1.560 |
| High resolution limit [Å] | 1.530 | 1.530 |
| Number of reflections | 51294 | 2518 |
| <I/σ(I)> | 14.3 | |
| Completeness [%] | 99.4 | |
| Redundancy | 12.7 | |
| CC(1/2) | 0.990 | 0.480 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 300 | 1.5 M Ammonium Sulphate 0.1 M Sodium citrate pH 4.2 |
