6TS2
Truncated version of Chaetomium thermophilum UDP-Glucose Glucosyl Transferase (UGGT) lacking domain TRXL2 (417-650).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2018-01-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 151.145, 191.014, 158.807 |
| Unit cell angles | 90.00, 117.70, 90.00 |
Refinement procedure
| Resolution | 140.600 - 5.740 |
| R-factor | 0.1778 |
| Rwork | 0.174 |
| R-free | 0.24890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nv4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 140.609 | 6.152 |
| High resolution limit [Å] | 5.740 | 5.740 |
| Rmerge | 0.150 | 1.432 |
| Rmeas | 0.165 | 1.569 |
| Rpim | 0.069 | 0.636 |
| Number of reflections | 17344 | 817 |
| <I/σ(I)> | 3.6 | 1.3 |
| Completeness [%] | 91.5 | |
| Redundancy | 5.6 | |
| CC(1/2) | 0.991 | 0.467 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.85 | 291 | 0.1M Sodium citrate pH 5.5, 20% w/v PEG 3,000 |
