6TS2

Truncated version of Chaetomium thermophilum UDP-Glucose Glucosyl Transferase (UGGT) lacking domain TRXL2 (417-650).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-01-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	151.145, 191.014, 158.807
Unit cell angles	90.00, 117.70, 90.00

Refinement procedure

Resolution	140.600 - 5.740
R-factor	0.1778
Rwork	0.174
R-free	0.24890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nv4
RMSD bond length	0.009
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	140.609	6.152
High resolution limit [Å]	5.740	5.740
Rmerge	0.150	1.432
Rmeas	0.165	1.569
Rpim	0.069	0.636
Number of reflections	17344	817
<I/σ(I)>	3.6	1.3
Completeness [%]	91.5
Redundancy	5.6
CC(1/2)	0.991	0.467

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.85	291	0.1M Sodium citrate pH 5.5, 20% w/v PEG 3,000