6TS0
Crystal structure of human L ferritin (HuLf) triple variant E60A-E61A-E64A Fe(III)-loaded for 30 minutes
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-19 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97622 |
Spacegroup name | I 4 3 2 |
Unit cell lengths | 151.386, 151.386, 151.386 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.918 - 2.200 |
Rwork | 0.203 |
R-free | 0.24810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lg8 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.875 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 107.050 | 2.640 | 2.300 |
High resolution limit [Å] | 2.200 | 2.500 | 2.180 |
Rmerge | 0.067 | 1.564 | 1.513 |
Rmeas | 0.071 | 1.642 | 1.585 |
Rpim | 0.015 | 0.363 | 0.351 |
Number of reflections | 15371 | 1533 | 2272 |
<I/σ(I)> | 31.7 | 2 | 2.2 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 21 | 20.2 | 20 |
CC(1/2) | 1.000 | 0.767 | 0.744 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 80 mM cadmium nitrate, 80 mM ammonium sulfate, 200 mM sodium acetate, pH 4.6 |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 80 mM cadmium nitrate, 80 mM ammonium sulfate, 200 mM sodium acetate, pH 4.6 |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 80 mM cadmium nitrate, 80 mM ammonium sulfate, 200 mM sodium acetate, pH 4.6 |