6TRO
Crystal structure of the T-cell receptor GVY01 bound to HLA A2*01-GVYDGREHTV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 220.040, 220.040, 96.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 67.910 - 3.000 |
| R-factor | 0.2162 |
| Rwork | 0.213 |
| R-free | 0.27020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3huj 6avf 5e00 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.203 |
| Data reduction software | xia2 (0.5.744) |
| Data scaling software | xia2 (0.5.744) |
| Phasing software | PHASER (2.7.17) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 67.910 | 67.930 | 3.050 |
| High resolution limit [Å] | 3.000 | 8.140 | 3.000 |
| Rmeas | 0.102 | 0.034 | 1.939 |
| Rpim | 0.037 | 0.013 | 0.687 |
| Total number of observations | 183925 | 8722 | 9402 |
| Number of reflections | 27736 | 1455 | 1382 |
| <I/σ(I)> | 13.5 | 38.9 | 1.2 |
| Completeness [%] | 98.6 | 92.9 | 99.6 |
| Redundancy | 6.6 | 6 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1 M succinic acid, 0.1 M HEPES pH 7.0, 1 % w/v PEG MME 2k |
