6TRK
Phl p 6 fold stabilized mutant - S46Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 47.825, 73.515, 76.124 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.800 - 1.600 |
| R-factor | 0.1571 |
| Rwork | 0.156 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nlx |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.993 |
| Data reduction software | SCALA |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.940 | 1.690 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 34555 | 5013 |
| <I/σ(I)> | 9.3 | 2.3 |
| Completeness [%] | 96.9 | 97.5 |
| Redundancy | 3.5 | 3.1 |
| CC(1/2) | 0.998 | 0.778 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50 mM Zinc acetate dehydrate, 10% (w/v) PEG 3350 |
