6TQB

X-ray structure of Roquin ROQ domain in complex with a UCP3 CDE1 SL RNA motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-14
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	86.990, 86.990, 72.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.080 - 1.600
R-factor	0.1582
Rwork	0.156
R-free	0.19720
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4qi2
RMSD bond length	0.013
RMSD bond angle	1.836
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.640
High resolution limit [Å]	1.600	1.600
Rmerge	0.047	0.736
Number of reflections	36355	2053
<I/σ(I)>	23.16	2.46
Completeness [%]	96.9	75.5
Redundancy	8.4	6.3
CC(1/2)	1.000	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	0.01 M Bis Tris Propane pH 6.5, 0.29 M Sodium Tartrate, 19 % PEG 3350