6TQA
X-ray structure of Roquin ROQ domain in complex with a UCP3 CDE2 SL RNA motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-13 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.530, 160.660, 68.010 |
| Unit cell angles | 90.00, 107.96, 90.00 |
Refinement procedure
| Resolution | 42.400 - 2.400 |
| R-factor | 0.2001 |
| Rwork | 0.197 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qi0 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.796 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.010 | 0.837 |
| Number of reflections | 35375 | 2623 |
| <I/σ(I)> | 14.05 | 2.15 |
| Completeness [%] | 99.6 | 99.8 |
| Redundancy | 5.2 | 5.1 |
| CC(1/2) | 1.000 | 0.630 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 250 mM ammonium sulphate and 30% (v/w) PEG 4000 |
