6TPG

Crystal structure of the Orexin-2 receptor in complex with EMPA at 2.74 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.96861
Spacegroup name	C 2 2 21
Unit cell lengths	91.097, 172.914, 77.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.548 - 2.741
R-factor	0.2203
Rwork	0.218
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5wqc
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.550	2.980
High resolution limit [Å]	2.740	2.740
Number of reflections	11345	475
<I/σ(I)>	6.7
Completeness [%]	89.1
Redundancy	8
CC(1/2)	0.977	0.346

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	100 mM trisodium citrate buffer 150-300 mM sodium chloride 28-43 % (v/v) polyethylene glycol 400