6TP5

Crystal structure of human Transmembrane prolyl 4-hydroxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976
Spacegroup name	P 31
Unit cell lengths	92.079, 92.079, 129.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.300 - 2.250
R-factor	0.1829
Rwork	0.181
R-free	0.22130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jig
RMSD bond length	0.005
RMSD bond angle	0.696
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.300	2.330
High resolution limit [Å]	2.250	2.250
Rmerge	0.092	1.950
Rmeas	0.109	2.380
Rpim	0.057	1.340
Number of reflections	57041	4799
<I/σ(I)>	7.35	0.4
Completeness [%]	98.0	83
Redundancy	3.4	2.7
CC(1/2)	0.995	0.180

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	277	Well solution: 0.1 M Tris-HCl pH 9, 22% Tert-butanol, 1 mM N-oxalylglycine Protein solution: 10 mM Tris-HCl pH 7.8, 0.1 M NaCl, 0.1 M Glycine, 2 mM CaCl2, 0.02 mM FeSO4