6TLF
human 14-3-3 sigma isoform in complex with IMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9159 |
| Spacegroup name | I 21 21 21 |
| Unit cell lengths | 63.294, 100.896, 157.017 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.630 - 2.900 |
| R-factor | 0.2088 |
| Rwork | 0.206 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yz5 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.990 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.880 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.104 | 0.928 |
| Rmeas | 0.116 | 1.033 |
| Rpim | 0.050 | |
| Number of reflections | 11508 | 1638 |
| <I/σ(I)> | 10.1 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 5.2 | 5.1 |
| CC(1/2) | 0.998 | 0.727 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 281 | 40 % wt/vol PEG 4000, 600mM ammonium sulfate, 100 mM Tris-HCl, pH 9.0 |
