6TKZ

Crystal structure of the DHR2 domain of DOCK10 in complex with CDC42

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2009-09-09
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.92
Spacegroup name	P 41 21 2
Unit cell lengths	97.170, 97.170, 312.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	82.480 - 2.640
R-factor	0.2153
Rwork	0.212
R-free	0.27440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wm9
RMSD bond length	0.009
RMSD bond angle	1.001
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (dev_4620)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.480	2.740
High resolution limit [Å]	2.600	2.600
Number of reflections	45143	6562
<I/σ(I)>	6.47
Completeness [%]	99.9
Redundancy	7.76

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.5	293	25% (w/v) PEG 3350, 200 mM potassium acetate, 8% (v/v) 1,1,1,3,3,3-Hexafluoro-2-propanol.