6TKV

Crystal structure of the human FUT8 in complex with GDP and a biantennary complex N-glycan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-24
Detector	DECTRIS PILATUS3 X 1M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	208.039, 68.589, 173.921
Unit cell angles	90.00, 149.90, 90.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.1868
Rwork	0.185
R-free	0.21884
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2de0
RMSD bond length	0.016
RMSD bond angle	2.065
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.010	2.060
High resolution limit [Å]	1.950	1.950
Rmerge	0.133	1.368
Number of reflections	89000	12914
<I/σ(I)>	6.4	1.4
Completeness [%]	99.9
Redundancy	4.4
CC(1/2)	0.995	0.447

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M Carboxilic acids 0.1 M Buffer system 3 pH 8.5 30% Precipitant mix 1 (Molecular Dimensions).