Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.979500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.990, 76.760, 148.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.560 - 2.750 |
| Rwork | 0.221 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nob |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.396 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Number of reflections | 12895 | 952 |
| <I/σ(I)> | 5.66 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.397 | |
| CC(1/2) | 0.990 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295.15 | 7.5-25% PEG6000, Bicine pH=9.0 |
