6THG
Cedar Virus attachment glycoprotein (G) in complex with human ephrin-B1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-09 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 112.796, 138.435, 235.394 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 69.218 - 4.074 |
R-factor | 0.2778 |
Rwork | 0.276 |
R-free | 0.31410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6thb |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.170 | 4.140 |
High resolution limit [Å] | 4.070 | 4.070 |
Rmerge | 0.407 | 2.192 |
Rpim | 0.173 | 0.914 |
Number of reflections | 30120 | 1373 |
<I/σ(I)> | 3.4 | 0.9 |
Completeness [%] | 99.8 | 92.4 |
Redundancy | 6.5 | |
CC(1/2) | 0.965 | 0.359 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25% polyethylene glycol 1500 (w/v), 0.1 M PCB (sodium propionate, sodium cacodylate, and BIS-TRIS propane) pH 5.0, and 0.02 M cadmium chloride dihydrate. |