6TG8
Crystal structure of the Kelch domain in complex with 11 amino acid peptide (model of the ETGE loop)
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 5.2R |
Synchrotron site | ELETTRA |
Beamline | 5.2R |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-04 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.296, 75.296, 127.539 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 17.000 - 2.750 |
Rwork | 0.181 |
R-free | 0.25840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zgk |
RMSD bond length | 0.007 |
RMSD bond angle | 1.499 |
Data reduction software | CrysalisPro (1.171.40.53) |
Data scaling software | Aimless (7.0.077) |
Phasing software | AMoRE (7.0.077) |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 65.208 | 2.800 |
High resolution limit [Å] | 2.450 | 2.700 |
Rmerge | 0.110 | 0.614 |
Number of reflections | 11948 | 1198 |
<I/σ(I)> | 40.66 | |
Completeness [%] | 99.5 | |
Redundancy | 18.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289 | 1.8 M sodium acetate pH 7.0, 0.1 M Bis-Tris propane pH 7.0 |