6TFL

Lsm protein (SmAP) from Halobacterium salinarum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9724
Spacegroup name	P 41 21 2
Unit cell lengths	156.450, 156.450, 106.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.397
R-factor	0.1805
Rwork	0.178
R-free	0.23060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ljo
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MoRDa
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.483
High resolution limit [Å]	2.397	2.397
Rmerge	0.261	0.908
Rmeas	0.061	0.937
Rpim	0.069	0.232
Number of reflections	1318697	82803
<I/σ(I)>	14	2.34
Completeness [%]	99.8	98.38
Redundancy	19.9	15
CC(1/2)	0.996	0.891

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	15% PEG 8000; 100mM MES pH=6.5; 200mM calcium acetate