6TEP
Crystal structure of a galactokinase from Bifidobacterium infantis in complex with ADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.272, 164.495, 115.873 |
| Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
| Resolution | 94.580 - 1.450 |
| R-factor | 0.1871 |
| Rwork | 0.184 |
| R-free | 0.24490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1pie |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.939 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.580 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.095 | 0.663 |
| Number of reflections | 336915 | 16117 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 98.4 | 95.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.0, 0.15 - 0.25 M NaCl and 18 - 22 % (w/v) polyethylene glycol 6000). |
