6TEP
Crystal structure of a galactokinase from Bifidobacterium infantis in complex with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-12-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.272, 164.495, 115.873 |
Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
Resolution | 94.580 - 1.450 |
R-factor | 0.1871 |
Rwork | 0.184 |
R-free | 0.24490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1pie |
RMSD bond length | 0.016 |
RMSD bond angle | 1.939 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 94.580 | 1.470 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.095 | 0.663 |
Number of reflections | 336915 | 16117 |
<I/σ(I)> | 7.3 | |
Completeness [%] | 98.4 | 95.6 |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.0, 0.15 - 0.25 M NaCl and 18 - 22 % (w/v) polyethylene glycol 6000). |