6TEN

Crystal structure of Dot1L in complex with an inhibitor (compound 11).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-28
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.99994
Spacegroup name	P 63
Unit cell lengths	158.475, 158.475, 74.799
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	45.750 - 2.210
R-factor	0.18
Rwork	0.179
R-free	0.20100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5drt
RMSD bond length	0.010
RMSD bond angle	0.990
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.750	45.750	2.270
High resolution limit [Å]	2.210	9.880	2.210
Rmerge	0.099	0.048	1.278
Rmeas	0.104	0.050	1.345
Total number of observations	555646
Number of reflections	53796	634	4000
<I/σ(I)>	14.82	42.36	2.12
Completeness [%]	100.0	98.8	100
Redundancy	10.329	10.476	10.276

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	1.5 M Li sulfate 0.1 M Hepes pH 7.0