6TE6
Crystal structure of Dot1L in complex with an inhibitor (compound 3).
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.99999 |
Spacegroup name | P 63 |
Unit cell lengths | 158.540, 158.540, 74.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 45.770 - 1.980 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5drt |
RMSD bond length | 0.010 |
RMSD bond angle | 0.930 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.7) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.770 | 45.770 | 2.030 |
High resolution limit [Å] | 1.980 | 8.850 | 1.980 |
Rmerge | 0.063 | 0.019 | 1.047 |
Rmeas | 0.066 | 0.020 | 1.104 |
Total number of observations | 755867 | ||
Number of reflections | 74086 | 878 | 5431 |
<I/σ(I)> | 22.94 | 81.75 | 2.37 |
Completeness [%] | 99.9 | 99.1 | 99.5 |
Redundancy | 10.203 | 10.361 | 10.008 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 293 | 1.28 M Na/K-tartrate 0.1 M Hepes pH 6.6 |