6T6J

Crystal Structure of the C-terminal domain of the HIV-1 Integrase (subtype A2, mutant N254K, K340Q)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	85.306, 48.835, 66.749
Unit cell angles	90.00, 110.37, 90.00

Refinement procedure

Resolution	41.679 - 2.003
R-factor	0.1716
Rwork	0.166
R-free	0.22030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6t6i
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_3311)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.679	2.080
High resolution limit [Å]	2.000	2.000
Number of reflections	17471	1697
<I/σ(I)>	15.15
Completeness [%]	99.7
Redundancy	6.5
CC(1/2)	0.998	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M MES pH 6.5, 1M sodium malonate