6T5K
ECV-1 from Echinicola vietnamensis. Environmental metallo-beta-lactamases exhibit high enzymatic activity under zinc deprivation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-3 |
Synchrotron site | ESRF |
Beamline | MASSIF-3 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-22 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 0.9677 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 51.926, 65.676, 128.501 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.330 |
R-factor | 0.1486 |
Rwork | 0.147 |
R-free | 0.18350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1znb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.112 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 1.360 |
High resolution limit [Å] | 1.330 | 1.330 |
Rmerge | 0.094 | 1.300 |
Rpim | 0.064 | 0.671 |
Number of reflections | 49538 | 2951 |
<I/σ(I)> | 7.8 | 1 |
Completeness [%] | 97.1 | |
Redundancy | 5.3 | |
CC(1/2) | 0.998 | 0.413 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 25-26% PEG3350, ), 0.1 M BIS-TRIS buffer pH 6 and 0.2 M sodium acetate |