6T4G
ROR(gamma)t ligand binding domain in complex with cholesterol and allosteric ligand FM26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.976254 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 108.635, 108.635, 107.669 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.830 - 1.930 |
Rwork | 0.178 |
R-free | 0.21310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6sal |
RMSD bond length | 0.016 |
RMSD bond angle | 1.866 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 70.830 | 70.880 | 1.960 |
High resolution limit [Å] | 1.930 | 5.240 | 1.930 |
Number of reflections | 28798 | 1629 | 1389 |
<I/σ(I)> | 40.3 | ||
Completeness [%] | 100.0 | ||
Redundancy | 77.8 | ||
CC(1/2) | 1.000 | 1.000 | 0.923 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.2M AmSO4 + 0.1M Tris |