6T4B
CRYSTAL STRUCTURE OF HUMAN TDP-43 N-TERMINAL DOMAIN AT 2.55 A RESOLUTION
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.637, 95.224, 157.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.900 - 2.550 |
| R-factor | 0.2265 |
| Rwork | 0.225 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5mdi |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.257 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.900 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Rmerge | 0.156 | 0.986 |
| Number of reflections | 16549 | 1212 |
| <I/σ(I)> | 5.7 | 1.6 |
| Completeness [%] | 98.7 | 99.1 |
| Redundancy | 3.9 | 4 |
| CC(1/2) | 0.980 | 0.549 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.2M Sodium bromide, 0.1M Bis-Tris propane 6.5, 20% PEG 3350 |
