6T2S
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 156.620, 156.620, 197.051 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 135.637 - 3.300 |
| Rwork | 0.199 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.026 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 197.050 | 3.560 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Number of reflections | 22156 | 4469 |
| <I/σ(I)> | 5.1 | 1.7 |
| Completeness [%] | 100.0 | |
| Redundancy | 21.2 | |
| CC(1/2) | 0.992 | 0.679 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 1 M lithuim chloride, 1 M sodium citrate pH 4.0 and 20% PEG 6000 |
