6T2P
Prominent members of the human gut microbiota express endo-acting O-glycanases to initiate mucin breakdown
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-21 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9159 |
Spacegroup name | P 41 |
Unit cell lengths | 82.724, 82.724, 121.682 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 82.720 - 2.100 |
Rwork | 0.186 |
R-free | 0.21090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3iln |
RMSD bond length | 0.010 |
RMSD bond angle | 1.589 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.720 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 47588 | 3811 |
<I/σ(I)> | 8.7 | 1.6 |
Completeness [%] | 99.7 | |
Redundancy | 6.8 | |
CC(1/2) | 0.996 | 0.557 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 450 mM sodium malonate pH 7.0, 24% PEG3350 |