6T1Z
LmrP from L. lactis, in an outward-open conformation, bound to Hoechst 33342
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-10-09 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97166 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 104.760, 139.990, 115.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.900 |
R-factor | 0.225 |
Rwork | 0.225 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | In-house structure |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.875 | 2.980 |
High resolution limit [Å] | 2.900 | 2.900 |
Number of reflections | 12755 | 281 |
<I/σ(I)> | 13.36 | |
Completeness [%] | 66.4 | |
Redundancy | 21 | |
CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 80 mM SPG pH 7, 20% (w/v) PEG 1500 |