6T12

Crystal structure of YTHDC1 with fragment 30 (DHU_DC1_220)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-16
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.947, 103.461, 42.467
Unit cell angles	90.00, 105.17, 90.00

Refinement procedure

Resolution	40.990 - 1.460
R-factor	0.1765
Rwork	0.175
R-free	0.19570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3h
RMSD bond length	0.005
RMSD bond angle	0.819
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.000	1.400
High resolution limit [Å]	1.320	1.320
Number of reflections	135409	13146
<I/σ(I)>	12.44
Completeness [%]	87.0
Redundancy	1.77
CC(1/2)	0.999	0.394

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG3350, 0.2M Ammonium Sulphate, 0.1M bis-tris, pH 6.5