6SY7
Structure of Trypanosome Brucei Phosphofructokinase in complex with AMP.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9159 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.395, 133.711, 271.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.810 - 2.750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f5m |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.840 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.810 | 2.848 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.075 | 0.604 |
| Rmeas | 0.100 | 0.808 |
| Rpim | 0.398 | |
| Number of reflections | 111370 | 5467 |
| <I/σ(I)> | 9.6 | 1.5 |
| Completeness [%] | 99.0 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.997 | 0.694 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | PEG 8000, sodium cacodylate |
