6SWU
Crystal structure of the TPR domain of KLC1 in complex with an engineered high-affinity cargo peptide.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-23 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 101.732, 107.532, 222.650 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 111.325 | 2.900 |
High resolution limit [Å] | 2.849 | 2.850 |
Rmerge | 0.114 | 1.543 |
Rmeas | 0.131 | 1.791 |
Rpim | 0.063 | 0.889 |
Number of reflections | 57529 | 2758 |
<I/σ(I)> | 8 | 1.2 |
Completeness [%] | 99.3 | 97.1 |
Redundancy | 4.47 | 4.42 |
CC(1/2) | 0.994 | 0.365 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 300 | 0.1 M MIB buffer pH 9.0, 25% PEG 1500 |