6SVQ

Crystal structure of human GFAT-1 G461E after UDP-GlcNAc soaking

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-12
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.97
Spacegroup name	P 41 21 2
Unit cell lengths	152.453, 152.453, 164.904
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.969 - 2.717
R-factor	0.2059
Rwork	0.205
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
RMSD bond length	0.002
RMSD bond angle	0.440
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XDS (Jan 26, 2018)
Phasing software	PHENIX (dev_2499)
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.969	2.810
High resolution limit [Å]	2.717	2.717
Number of reflections	52752	5146
<I/σ(I)>	16.86
Completeness [%]	99.7
Redundancy	8.8
CC(1/2)	0.999	0.549

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.75, 0.35 M Potassium sodium tartrate, 20 % PEG3350