6SVO

Crystal structure of human GFAT-1 in complex with Glucosamine-6-Phosphate and L-Glu

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	80
Detector technology	PIXEL
Collection date	2019-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.976300
Spacegroup name	P 41 21 2
Unit cell lengths	152.848, 152.848, 165.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.335 - 2.328
R-factor	0.1908
Rwork	0.190
R-free	0.22400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
RMSD bond length	0.002
RMSD bond angle	0.463
Data reduction software	XDS (Mar 15, 2019)
Data scaling software	XDS (Mar 15, 2019)
Phasing software	PHENIX (dev_2499)
Refinement software	PHENIX (dev_2499)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.335	2.410
High resolution limit [Å]	2.328	2.330
Number of reflections	84017	8181
<I/σ(I)>	18.8
Completeness [%]	99.8
Redundancy	22.3
CC(1/2)	0.999	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 8.75, 0.2 M Potassium sodium tartrate, 20 % PEG3350