6SVL
human Myeloid-derived growth factor (MYDGF) in complex with neutralizing Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999890 |
| Spacegroup name | P 1 |
| Unit cell lengths | 89.519, 107.430, 109.369 |
| Unit cell angles | 71.54, 86.25, 73.38 |
Refinement procedure
| Resolution | 97.820 - 1.580 |
| R-factor | 0.182 |
| Rwork | 0.181 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1iqw |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.820 | 1.584 |
| High resolution limit [Å] | 1.580 | 1.584 |
| Rmerge | 0.052 | 0.633 |
| Number of reflections | 320264 | 160008 |
| <I/σ(I)> | 10.2 | 1.8 |
| Completeness [%] | 63.5 | |
| Redundancy | 2.7 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 277.15 | 0.1 M trisodium citrate (pH 5.5) and 20 % PEG 3000 |
