6SV2
Human prion protein (PrP) fragment 119-231 (G127V M129 variant) complexed to ICSM 18 (anti-Prp therapeutic antibody) Fab fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 300 |
| Detector technology | PIXEL |
| Collection date | 2013-02-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 128.076, 128.076, 135.642 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 57.930 - 2.300 |
| R-factor | 0.2026 |
| Rwork | 0.200 |
| R-free | 0.25120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2w9e |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.735 |
| Data reduction software | XDS |
| Data scaling software | pointless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.930 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rpim | 0.103 | 1.224 |
| Number of reflections | 26908 | 2626 |
| <I/σ(I)> | 24.5 | 2.7 |
| Completeness [%] | 88.5 | |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.8 M and 1.5 M ammonium sulphate, 0.1 M Tris (pH 7.5 and 8.0) |
