6SUV
Horse cytochrome c complexed by octa-anionic calixarene
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-02-20 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 43 |
| Unit cell lengths | 65.591, 65.591, 250.698 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.380 - 2.502 |
| Rwork | 0.170 |
| R-free | 0.23660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 3.346 |
| Data reduction software | DENZO |
| Data scaling software | Aimless |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.380 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.145 | 0.316 |
| Rmeas | 0.178 | 0.400 |
| Rpim | 0.101 | 0.243 |
| Number of reflections | 34903 | 3775 |
| <I/σ(I)> | 5.9 | 2.6 |
| Completeness [%] | 96.3 | 92.6 |
| Redundancy | 2.7 | 2.4 |
| CC(1/2) | 0.943 | 0.739 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 56% PEG 3350, 0.05 M sodium cacodylate pH 5.5 and 0.0017 M gadolinium chloride |
