6SUR
The Rab33B-Atg16L1 crystal structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2016-05-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.400, 204.900, 107.200 |
Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
Resolution | 48.350 - 3.467 |
R-factor | 0.2073 |
Rwork | 0.206 |
R-free | 0.23880 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z06 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.350 | 48.350 | 3.600 |
High resolution limit [Å] | 3.467 | 47.100 | 3.470 |
Rmerge | 0.194 | 0.058 | 0.750 |
Rmeas | 0.229 | 0.081 | 0.892 |
Number of reflections | 25983 | 3 | 2227 |
<I/σ(I)> | 7.86 | 14.28 | 2.2 |
Completeness [%] | 96.3 | 27.3 | 79.9 |
Redundancy | 3.466 | 1.667 | 3.282 |
CC(1/2) | 0.983 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES monohydrate pH 6.5, 0.2 M sodium chloride, 10 % (w/v) PEG 4 000 0.1 M spermine |