6STY

Human REXO2 exonuclease in complex with RNA.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-29
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.89440
Spacegroup name	P 32
Unit cell lengths	88.303, 88.303, 123.773
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.110 - 3.150
R-factor	0.1988
Rwork	0.163
R-free	0.22170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cy4
RMSD bond length	0.003
RMSD bond angle	0.531
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.110	3.340
High resolution limit [Å]	3.150	3.150
Number of reflections	18669	2940
<I/σ(I)>	14.81	3.06
Completeness [%]	99.4	96.8
Redundancy	5.1	4.9
CC(1/2)	0.994	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291.15	0.2 M potassium sodium tartrate trihydrate and 20% PEG3350