6SRF

Crystal Structure of Human Prolidase G278N variant expressed in the presence of chaperones

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	C 2 2 21
Unit cell lengths	103.557, 106.692, 216.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.860 - 1.847
R-factor	0.181
Rwork	0.180
R-free	0.21650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m4g
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.860	1.960
High resolution limit [Å]	1.847	1.850
Rmerge	0.171	1.622
Number of reflections	102309	16073
<I/σ(I)>	10.8	1.03
Completeness [%]	99.5	97.6
Redundancy	7.33	1.02
CC(1/2)	0.997	0.479

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	10mM NaBorate, 690-760mM NaCitrate