6SQX

Insights into a novel NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 21
Unit cell lengths	76.578, 109.966, 130.936
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.290 - 1.400
R-factor	0.1481
Rwork	0.147
R-free	0.17540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3m1u
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.300	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.046	1.470
Rmeas	0.051	1.626
Rpim	0.022	0.690
Number of reflections	216685	21426
<I/σ(I)>	18.86
Completeness [%]	99.8	99.6
Redundancy	5.5	5.4
CC(1/2)	1.000	0.616

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	100 mM Imidazole pH 8.0 + 15 % PEG 8K