6SQX
Insights into a novel NlpC/P60 Endopeptidase from Photobacterium damselae subsp. piscicida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-02-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.578, 109.966, 130.936 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.290 - 1.400 |
R-factor | 0.1481 |
Rwork | 0.147 |
R-free | 0.17540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3m1u |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.300 | 1.450 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.046 | 1.470 |
Rmeas | 0.051 | 1.626 |
Rpim | 0.022 | 0.690 |
Number of reflections | 216685 | 21426 |
<I/σ(I)> | 18.86 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 5.5 | 5.4 |
CC(1/2) | 1.000 | 0.616 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 100 mM Imidazole pH 8.0 + 15 % PEG 8K |