6SQN

Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant co-crystallized with RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-01
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03320
Spacegroup name	P 65 2 2
Unit cell lengths	96.938, 96.938, 258.730
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.520 - 2.050
R-factor	0.2306
Rwork	0.230
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1urn
RMSD bond length	0.010
RMSD bond angle	1.661
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.740	2.090
High resolution limit [Å]	2.050	2.050
Rmerge	0.199	2.383
Rmeas	0.201	2.414
Number of reflections	49417	2231
<I/σ(I)>	18.2
Completeness [%]	100.0	99.9
Redundancy	38	39.6
CC(1/2)	0.999	0.683

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.2 M ammonium sulfate, 0.1 M tri-potassium citrate