6SPO
Structure of the Escherichia coli methionyl-tRNA synthetase complexed with methionine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-04-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.290, 45.190, 85.890 |
Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
Resolution | 33.050 - 1.200 |
R-factor | 0.138 |
Rwork | 0.136 |
R-free | 0.16730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qqt |
RMSD bond length | 0.008 |
RMSD bond angle | 0.949 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.100 | 1.270 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 178894 | 29243 |
<I/σ(I)> | 10 | 1.11 |
Completeness [%] | 99.0 | 94.8 |
Redundancy | 6.4 | |
CC(1/2) | 0.998 | 0.326 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 281 | 1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0 |