6SPO
Structure of the Escherichia coli methionyl-tRNA synthetase complexed with methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 78.290, 45.190, 85.890 |
| Unit cell angles | 90.00, 107.39, 90.00 |
Refinement procedure
| Resolution | 33.050 - 1.200 |
| R-factor | 0.138 |
| Rwork | 0.136 |
| R-free | 0.16730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qqt |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.949 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.100 | 1.270 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Number of reflections | 178894 | 29243 |
| <I/σ(I)> | 10 | 1.11 |
| Completeness [%] | 99.0 | 94.8 |
| Redundancy | 6.4 | |
| CC(1/2) | 0.998 | 0.326 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 281 | 1.08 M ammonium citrate, 10 mM potassium phosphate, pH 7.0 |
